Custom Real-Time PCR (qPCR)
Our Real-Time PCR (qPCR) service encompasses the complete workflow, from primer design to PCR data analysis.
We work closely with you to tailor the process to your specific needs and make any adjustments needed to achieve the best possible results.
Quantitative PCR (qPCR), also known as real-time PCR, is a powerful and widely-used molecular biology technique that enables the precise detection and quantification of nucleic acids. It has revolutionized research and diagnostic workflows across fields such as gene expression analysis, pathogen detection, and genetic variation studies. qPCR combines the specificity and amplification efficiency of traditional PCR with real-time detection, providing a reliable and highly sensitive approach to monitor DNA or RNA in diverse applications. This is made possible by state-of-the-art
instruments housed in our labs, including the CFX 96, QuantStudio 5-DX™, CFS Opus 96 - Dx, and Fluidigm Biomark™ HD.
qPCR offers exceptional sensitivity, specificity, and reproducibility, making it ideal for detecting low-abundance targets, quantifying gene expression, and monitoring genetic variations.
Whether you are engaged in genomics research, oncology studies, or infectious disease diagnostics, we deliver comprehensive real-time PCR (qPCR) services that provide the reliable data you need to advance your scientific endeavors.
Our product portfolio
Our experts leverage cutting-edge technologies in our molecular biology labs to perform real-time PCR (qPCR) experiments with high sensitivity, specificity, and reproducibility.
CFX 96
QS5-DX™
CFS Opus 96 - Dx
Fluidigm Biomark™ HD
Number of samples per assay
96 samples/assay
96 samples/assay
96 samples/assay
96 samples/assay
RUO
IVDR
real-time pcr (qPCR) principle
The principle of qPCR lies in the continuous monitoring of DNA amplification during the reaction, achieved through the use of fluorescent dyes or probes.
Amplification:
Like conventional PCR, qPCR involves repeated cycles of denaturation, annealing, and extension to amplify a target DNA sequence. If the starting material is RNA, a reverse transcription step is performed first to generate complementary DNA (cDNA).
Fluorescence Detection:
• Intercalating Dyes: Dyes such as SYBR Green bind to double-stranded DNA and emit fluorescence proportional to the amount of DNA generated.
• Probe-Based Detection: Probes like TaqMan contain a fluorescent reporter and quencher, providing target-specific fluorescence upon successful amplification.
Real-Time Quantification:
The fluorescence intensity is measured during each cycle, allowing the accumulation of DNA to be tracked in real time. The cycle at which fluorescence surpasses a set threshold (Ct value) reflects the initial quantity of the target DNA.
Quantification Methods:
qPCR can provide absolute quantification using standard curves, or relative quantification (comparing target expression to reference genes).
For more details on each system and its specific applications, connect with our experts today! We're here to help you find the perfect solution for your research or diagnostic needs.
Why Firalis Molecular Precision Is the Best Partner for Your Project
Our custom Real-Time PCR (qPCR) services are designed to meet the needs of researchers requiring precise and reliable gene expression analysis for their experiments.
Whether you are studying gene expression in cancer cells, investigating the role of specific genes in diseases through genetic variations such as SNPs or CNVs, profiling miRNA, or developing new molecular diagnostic assays, our services provide the accuracy and dependability necessary to achieve your research objectives.
Complete workflow
Our qPCR service covers the entire workflow, from the initial design of primers to the final analysis of PCR data. We collaborate closely with you to understand your specific needs and make any necessary adjustments to ensure optimal results.
Qualified Team
Our team boasts extensive experience and specialized training in qPCR. We provide comprehensive support throughout the entire process, from sample preparation to data analysis, ensuring a successful project outcome.
High-quality results
Our experts use advanced platforms to conduct qPCR experiments with exceptional sensitivity and specificity, delivering high-quality data. Our bioinformatics specialists then analyze this data to provide you with actionable insights and strategic recommendations.
OUR COMMITMENT
Timeliness
We are committed to timely delivery and efficient project execution, ensuring that your research progresses according to schedule
Tailored Solutions
We are dedicated to understanding your goals and providing customized services with proactive communication throughout the project.
Quality Assurance
We follow strict, accredited procedures to guarantee the accuracy and reliability of your data.
Innovation
Our specialists leverage advanced technologies and methodologies to deliver innovative solutions tailored to your project.
