Single-cell Sequencing
Explore gene expression profiles at a single cell level combining gene expression data with cell surface markers, TCR and BCR clone frequencies or open chromatin analysis.
Our single-cell sequencing service uses the latest technology from 10x Genomics® to provide high-quality single-cell sequencing data : the Chromium iX.
Our experts use two specific protocols: the 3’/5’ protocol and the Fixed Flex protocol, providing valuable insights into transcriptional mechanisms and gene regulation.
This diverse approach enables us to conduct precise analyses, shedding light on gene expression dynamics and RNA-regulated biological processes.
10x Genomics® Next GEM TECHNOLOGY PRINCIPLE
10x Genomics® Next GEM technology uses droplet-based microfluidics to partition individual cells into tiny droplets, where each droplet is loaded with unique barcoded primers that enable the identification of individual cells during sequencing.
This technology allows for high-throughput analysis of thousands of individual cells, enabling a more comprehensive understanding of cellular heterogeneity.
Illustration of the 10x Genomics® Next GEM Technology principle
EXPLORE OUR SERVICES
Sample preparation
We provide assistance with cell isolation, dissociation, and preservation to ensure high-quality single-cell suspensions for downstream sequencing.
Library preparation
We perform library preparation using the 10x Genomics platform, which includes cell encapsulation, barcoding, and cDNA synthesis. Our service includes quality control measures to ensure that only high-quality libraries are used for sequencing.
Sequencing
We offer high-throughput sequencing on Illumina® platforms to generate high-quality single-cell sequencing data.
Data analysis
We provide a comprehensive analysis of the single-cell sequencing data, including quality control, alignment, quantification of gene expression, and clustering analysis to identify cell subpopulations and marker genes.
What sets our Single-Cell Sequencing services apart ?
Comprehensive analysis of cellular heterogeneity
Our single-cell RNA Seq service enables a comprehensive analysis of cellular heterogeneity and thus, the identification of cell subpopulations and marker genes that would be missed in traditional bulk sequencing approaches.
Qualified team
Our experts in single-cell sequencing and omics data analysis will provide assistance throughout the entire sequencing process, from sample preparation to data analysis, to ensure a successful project outcome.
High-quality data
We use state-of-the-art technology and quality control measures to ensure high-quality data, which is critical for downstream analysis.