Large-scale Protein Identification with Olink®
We have been a trusted Olink® service provider for over ten years, and our proteomics team has extensive experience with different types of samples and Olink® platforms and panels.
Firalis Molecular Precision is a Certified Olink Service Provider and the only Service Provider in France proficient in using the Flex, Target 48 and Target 96 panels and Explore HT, and Explore 384-plex platforms for proteomics analysis.
Olink® Technology
Olink® technology is a powerful tool for studying protein expression, allowing a comprehensive understanding of biological processes.
Our service leverages two different Olink® panels – Target (96 and 48) and Flex – and Olink® Explore and Explore HT platforms
The protein detection is allowed by Proximity Extended Assay (PEA), a sensitive and high-throughput technology.
Proximity Extension Assay (PEA) works by using pairs of antibodies, each attached to unique DNA strands, to bind specific proteins in a sample. When both antibodies bind to the same protein, the DNA strands come into close proximity and hybridize. This hybridized DNA is then amplified using PCR, and the amount of DNA generated reflects the concentration of the target protein.
Olink® Explore platform
Our bioanalytical laboratories are equipped with SPT Labtech’s mosquito® Liquid Handling and dragonfly® discovery instruments to automate key steps when carrying out large-scale proteomics studies using the Olink Explore platform.
This high-throughput biomarker discovery platform uses NGS readout on Illumina® instruments and Olink’s proprietary Proximity Extension Assay (PEA) technology to measure thousands of human plasma proteins from small sample volumes.
The Olink® Explore 3072 provides access to the entire Olink® protein library of around 3000 assays, suitable for exploratory proteomics and multi-omics studies. Alternatively, for focused projects, one from the eight Explore 384-plex panels within the Explore 3072 library can be chosen.
3072 assay through 8 x 384-plex kits (customizable panel selection, running from 1 to 8 panels) :
- Olink® Explore 384 Cardiometabolic & Cardiometabolic II
- Olink® Explore 384 Inflammation & Inflammation II
- Olink® Explore 384 Neurology & Neurology II
- Olink® Explore 384 Oncology & Oncology II
Hear from our partners
“The combination of the Olink Explore platform with SPT Labtech automated true positive displacement liquid handling and Firalis Molecular Precision expertise enables swift and precise execution of large-scale proteomics studies, spanning all crucial biological pathways and therapeutic areas.
The ability to pipette ultra-low volumes without compromising accuracy supports reaction miniaturization, which in combination with low dead volumes, uncovers new scientific insights from as little as 2 µL sample.”
– Kamila Koprowska, PhD, Senior Business Development Scientist, SPT Labtech
Olink® Explore HT platform
A significant leap forward in next-generation proteomics
The disruptive Olink® Explore HT platform enables the analysis of large sample sets simultaneously, making it a scalable technology platform for high-volume proteomics research. It enables our experts to measure 5,400 protein biomarkers in only 2 µl of sample with accuracy, speed and quality, unveiling the human proteome in the blink of an eye and providing you with deeper biological insights.
On-demand Webinar
In this webinar, we discuss how combining Olink® Explore with SPT Labtech’s positive displacement liquid handling technology is revolutionizing large-scale proteomic data generation for biomarker discovery. We dive deep into how this integration supports high-throughput research and provides deeper insights into the physiopathologic mechanisms underlying various drugs and diseases.
Key Learning Objectives:
• Gain insights into next-generation proteomics
• Learn how to identify critical biomarkers for diagnostic, prognostic, and monitoring purposes
• Find out more about data integration
Olink® Target panel
The Target 96 & 48 panels offer high-multiplex immunoassays designed to target specific biological processes, disease areas, or pathways, making them ideal for focused protein biomarker discovery.
By enabling the simultaneous identification of multiple proteins, these panels allow for the creation of comprehensive protein signatures, facilitating faster and more informed decisions in biomarker research. This approach ensures superior data quality and performance, offering a more robust alternative to single-protein analyses for deeper insights into human health and disease.
Proteomics analyses using the Olink® Target 48 panels are conducted in our labs under GCLP conditions.
Olink® Target 96
- Limit of detection below pg/mL with only 1 µL of sample per panel.
- 15 disease-specific panels with more than 1100 markers.
- 92 human proteins biomarkers measured simultaneously, up to 88 samples in one panel.
- 14 human disease-focused and 1 panel for Mouse exploratory research available.
Olink® Target 48
- Provides both absolute and relative protein concentration reported in pg/mL or NPX units.
- Measurement of 45 well-established, inflammation-related and immune-related human protein biomarkers simultaneously.
- Needs only 1 µL of sample.
Olink® Target 48 Mouse Cytokine
- Measures 43 biomarkers from just 1 µL of sample
- Monitor Inflammation and Immune Response.
- Carry out longitudinal studies on the same mouse (multiple blood samples), to avoid experimenting on a large number of mice.
Olink® Flex panel
Olink® Flex is a personalized product that allows the assembly of a biomarker panel comprising up to 21 human proteins.
This is made possible by the ability to select from a pool of approximately 200 pre-validated protein biomarkers, which can be combined in various combinations, letting you select specific proteins from Olink’s vast library to tailor your study to your exact needs.
Proteomics analyses using the Olink® Flex panel are conducted under GcLP conditions.
- Up to 15 to 21 assays per panel, each panel measures 40 samples
- Readout in absolute quantification
- 200 inflammation-focused protein biomarkers
Why Firalis Molecular Precision Is the Best Partner for Your Project
Qualified proteomics experts
Our team, with extensive experience and training in using Olink® technologies, is committed to delivering optimal service and support for your research project, in every therapeutic area.
Different biological matrices
Large-scale protein identification in various biological matrices: Serum, EDTA plasma, Citrate plasma, Heparin plasma
Multiplexed protein analysis
Olink® technology enables simultaneous measurement of hundreds of proteins with low input in a single sample, saving time and costs compared to traditional methods.
OUR COMMITMENT
Timeliness
We are committed to timely delivery and efficient project execution, ensuring that your research progresses according to schedule
Tailored Solutions
We are dedicated to understanding your goals and providing customized services with proactive communication throughout the project.
Quality Assurance
We follow strict, accredited procedures to guarantee the accuracy and reliability of your data.
Innovation
Our specialists leverage advanced technologies and methodologies to deliver innovative solutions tailored to your project.
