Digital PCR (dPCR)
Our experts leverage Thermo Fisher’s QuantStudio Absolute Q Digital PCR System as a bioanalytical technology to offer fast and precise nucleic acid quantification for various applications in life sciences research.
What is Digital PCR (dPCR)?
Digital PCR (dPCR) is an advanced, highly sensitive technique used for precise quantification of nucleic acids, including DNA and RNA.
Unlike traditional PCR methods, which rely on relative measurements, Digital PCR provides absolute quantification by partitioning a sample into thousands of individual reactions.
Each partition acts as a separate PCR reaction, and the presence or absence of a target sequence in each partition is detected, allowing for a more accurate and reliable measurement.
To analyze the data, we apply the Poisson method, which helps to estimate the concentration of target nucleic acids by modeling the distribution of positive reactions across the partitions.
Our experts use Thermo Fisher’s Applied Biosystems QuantStudio Absolute Q Digital PCR System
A cutting-edge platform known for its speed and accuracy, which allows our specialists to perform high-throughput quantification with minimal sample input.
With our +15 years of expertise, we make sure to leverage the platform efficiently to provide high-quality results for applications like gene expression analysis and mutation detection.
MAP technology principle
Microfluidic Array Plate (MAP) technology is a key component of the QuantStudio Absolute Q Digital PCR System.
It partitions the sample into numerous microchambers without the need for physical displacement or fluidic shearing, which results in superior consistency, reduced reagent waste, and improved volume precision, all of which contribute to more accurate quantification of nucleic acids.
Fig 1. Microfluidic Array Plate Technology
Conducting Digital PCR (dPCR)
Sample preparation and PCR Setup
Nucleic acids (DNA/RNA) are extracted from the sample, and the PCR reaction mix is prepared with primers, probes, and other reagents.
Sample
partitioning
The PCR mix is dispensed onto the Digital PCR chip or microfluidic array, dividing the sample into thousands of individual microchambers.
Amplification and data collection
Thermal cycling amplifies the target nucleic acid, followed by data collection, which detects positive and negative reactions in each microchamber.
Data
analysis
The Poisson method is applied to calculate the absolute quantity of the target nucleic acid, and the results are validated and reported.
Why Firalis Molecular Precision Is the Best Partner for Your Project
Customized Data Analysis
From rare target detection to complex quantification, we provide advanced modeling, tailored visualizations, and detailed reporting for accurate, actionable insights.
Complete Workflow
Our Digital PCR service encompasses the entire process, from designing primers to delivering the final analysis of your PCR data, which can be integrated with other omics data, helping you streamline operations and enhance the efficiency of your research or drug development process.
Qualified Team
Our team of PhD-level experts has in-depth knowledge and specialized training in Digital PCR.
OUR COMMITMENT
Timeliness
We are committed to timely delivery and efficient project execution, ensuring that your research progresses according to schedule
Tailored Solutions
We are dedicated to understanding your goals and providing customized services with proactive communication throughout the project.
Quality Assurance
We follow strict, accredited procedures to guarantee the accuracy and reliability of your data.
Innovation
Our specialists leverage advanced technologies and methodologies to deliver innovative solutions tailored to your project.
