Single-Cell RNA Sequencing
Our expert team uses Single-Cell RNA Sequencing to provide insights into transcriptional mechanisms and gene regulation of individual cells using 10x Genomics technology, for cell differentiation, therapeutic response, and biomarker discovery.
Single-Cell RNA Sequencing involves isolating individual cells from a sample and extracting their RNA to sequence it in order to obtain information on gene expression or genetic variations. This powerful approach provides a high-resolution view of cellular heterogeneity, revealing differences between cells that may be masked in bulk analyses. Our genomics specialists use the Chromium iX by 10x Genomics® for our Single-Cell RNA Sequencing solution.
This advanced platform allows our experts to explore gene expression profiles at a single-cell level, while integrating the data with cell
surface markers, TCR and BCR clone frequencies, and open chromatin analysis.
Our experts employ three specific protocols:
- The 3’ Protocol: Capturing the 3' end of mRNA transcripts to profile gene expression.
- The 5’ protocol: Focusing on the 5' end of mRNA transcripts to understand gene regulation and transcript structure.
- The Flex protocol to perform gene expression analysis across various sample preservation methods.
10x Genomics® GEM-X Technology Principle
10x Genomics® Technology uses droplet-based microfluidics to partition individual cells into tiny droplets, where each droplet is loaded with unique barcoded primers that enable the identification of individual cells during sequencing.
This technology allows for high-throughput analysis of thousands of individual cells, enabling a more comprehensive understanding of cellular heterogeneity.
Illustration of the 10x Genomics® GEM-X Technology Principle
AN END-TO-END SOLUTION
We provide all the resources and services to cover every stage of the process, ensuring that you can move from sample to comprehensive and actionable insights with complete ease.
Sample preparation
Our experts provide assistance with cell isolation, dissociation, and preservation to ensure high-quality single-cell suspensions for downstream sequencing.
Library preparation
We perform library preparation using the 10x Genomics iX platform, which includes cell encapsulation, barcoding, and cDNA synthesis. We perform Quality Control measures to ensure that only high-quality libraries are used for sequencing.
Sequencing
We leverage high-throughput sequencing systems by Illumina® to generate high-quality single-cell sequencing data.
Data Analysis
Our bioinformatics experts provide a precise, in-depth analysis of the Single-cell Sequencing data, including quality control, alignment, quantification of gene expression, and clustering analysis to identify cell subpopulations and marker genes.
Why Firalis Molecular Precision Is the Best Partner for Your Project
Comprehensive analysis
Our Single-Cell RNA Sequencing service enables a comprehensive analysis of cellular heterogeneity and thus, the identification of cell subpopulations and marker genes that would be missed in traditional bulk sequencing approaches.
Qualified team
Our experts in Single-Cell RNA Sequencing and data analysis will provide assistance throughout the entire sequencing process, from sample preparation to data analysis, to ensure a successful project outcome.
High-quality data
We use state-of-the-art technology and quality control measures to ensure high-quality data, which is critical for downstream analysis.
OUR COMMITMENT
Timeliness
We are committed to timely delivery and efficient project execution, ensuring that your research progresses according to schedule
Tailored Solutions
We are dedicated to understanding your goals and providing customized services with proactive communication throughout the project.
Quality Assurance
We follow strict, accredited procedures to guarantee the accuracy and reliability of your data.
Innovation
Our specialists leverage advanced technologies and methodologies to deliver innovative solutions tailored to your project.
